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[1]彭茹洁,赵少甫,王承潇,等.Plackett-Burman试验联用满意度函数-响应面法优化白及多糖絮凝纯化工艺[J].南京工业大学学报(自然科学版),2019,41(01):57-63.[doi:10.3969/j.issn.1671-7627.2019.01.010]
 PENG Rujie,ZHAO Shaofu,WANG Chengxiao,et al.Optimization of flocculating purification on Bleailla striata polysaecharide by desirability function and response surface methodology combined with Plackett-Burman test[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2019,41(01):57-63.[doi:10.3969/j.issn.1671-7627.2019.01.010]
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Plackett-Burman试验联用满意度函数-响应面法优化白及多糖絮凝纯化工艺()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
41
期数:
2019年01期
页码:
57-63
栏目:
出版日期:
2019-01-28

文章信息/Info

Title:
Optimization of flocculating purification on Bleailla striata polysaecharide by desirability function and response surface methodology combined with Plackett-Burman test
文章编号:
1671-7627(2019)01-0057-07
作者:
彭茹洁1赵少甫1王承潇2韩伟1
1.华东理工大学 药学院 上海市新药设计重点实验室,上海 200237; 2.昆明理工大学 生命科学与技术学院,云南 昆明 650500
Author(s):
PENG Rujie1ZHAO Shaofu1WANG Chengxiao2HAN Wei1
1.Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China; 2.School of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China
关键词:
白及多糖 絮凝纯化 满意度函数 响应面
Keywords:
Bleailla striata polysaecharide flocculating purification desirability function response surface methodology
分类号:
R284.2
DOI:
10.3969/j.issn.1671-7627.2019.01.010
文献标志码:
A
摘要:
以白及多糖粗提液为对象,考察絮凝剂ZTC-Ⅱ絮凝纯化工艺。在单因素试验的基础上,运用Plackett-Burman(PB)试验,选择出具有显著影响的因素。通过满意度函数与响应面相结合的分析方法,建立各影响因素的二次回归方程模型,得到白及多糖的最佳纯化条件:絮凝温度为48 ℃,絮凝剂ZTC-Ⅱ组分B用量为0.77 mL/g,组分A、B用量比为0.34:1,絮凝时间为1.5 h,浓缩比为1:12 g/mL。在此优化工艺下,白及多糖保留率为(92.32±0.34)%,脱蛋白率为(51.43±0.12)%。
Abstract:
Flocculating purification for crude polysaecharide extracting solution of Bleailla striata by ZTC-Ⅱ flocculant was studied.The main influence factors were selected by Plackett-Burman(PB)test on the basis of these single factors.From the results of response surface methodology combined with desirability function,the regression equation of each influencing factor was established.The optimum purification conditions of crude Bleailla striata polysaccharide were as follows:flocculation temperature of 48 ℃,dosage of component B of 0.77 mL/g,ratio of component A and B of 0.34:1,flocculation time of 1.5 h,and ratio of mass to volume of 1:12 g/mL.Under such optimized conditions,the retention rate of polysaccharide was(92.32±0.34)%,and deproteinizing rate was(51.43±0.12)%.

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备注/Memo

备注/Memo:
收稿日期:2017-07-14
基金项目:云南省科技重大专项(2018ZF011-2018110506)
作者简介:彭茹洁(1992—),女,E-mail:1439577341@qq.com; 韩伟(联系人),教授,E-mail:whan@ecust.edu.cn.
引用本文:彭茹洁,赵少甫,王承潇,等.Plackett-Burman试验联用满意度函数-响应面法优化白及多糖絮凝纯化工艺[J].南京工业大学学报(自然科学版),2019,41(1):57-63..
更新日期/Last Update: 2019-02-26