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[1]徐婷,王俊超,赵少甫,等.川芎中欧当归内酯A的提取纯化与结构表征[J].南京工业大学学报(自然科学版),2018,40(02):71-76.[doi:10.3969/j.issn.1671-7627.2018.02.012]
 XU Ting,WANG Junchao,ZHAO Shaofu,et al.Preparation and determination of levistolide A from Ligusticum chuanxiong Hort.[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2018,40(02):71-76.[doi:10.3969/j.issn.1671-7627.2018.02.012]
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川芎中欧当归内酯A的提取纯化与结构表征
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
40
期数:
2018年02期
页码:
71-76
栏目:
出版日期:
2018-03-20

文章信息/Info

Title:
Preparation and determination of levistolide A from Ligusticum chuanxiong Hort.
文章编号:
1671-7627(2018)02-0071-06
作者:
徐婷1王俊超1赵少甫1刘伟1韩伟12
1.华东理工大学 中药现代化工程中心,上海 200237; 2.上海市中医临床重点实验室,上海 201203
Author(s):
XU Ting1WANG Junchao1ZHAO Shaofu1LIU Wei1HAN Wei12
1.Engineering Center for Traditional Chinese Medicine Modernization,East China University of Science and Technology,Shanghai 200237,China; 2.Shanghai Key Laboratory of Traditional Chinese Clinical Medicine,Shanghai 201203,China
关键词:
川芎 肝纤维化 超声波提取 欧当归内酯A
Keywords:
Ligusticum chuanxiong Hort. liver fibrosis ultrasonic-assisted extraction levistolide A
分类号:
R284.2
DOI:
10.3969/j.issn.1671-7627.2018.02.012
文献标志码:
A
摘要:
以川芎中的内酯成分得率为指标,选取提取温度、提取时间、液固比、药材颗粒度以及水解pH 5个因素,在单因素考察基础上采用正交实验对提取工艺进行优化,继而分离得到单体化合物,最后用液相色谱-质谱(LC-MS)联用技术及核磁共振仪(NMR)对产物进行结构表征。结果表明,最佳提取工艺条件为:提取温度30 ℃,提取时间50 min,液固比15 mL/g,药材颗粒度>150~180 μm,水解pH=13.0,川芎内酯得率可达5.91%。在最佳工艺条件下制得的化合物进一步分离得到欧当归内酯A。
Abstract:
Factors affecting the extraction rate of lactone compounds from Ligusticum chuanxiong Hort.,such as extractive temperature and duration,the liquid to solid ratio,granularity and pH value of solvent,were investigated.The lactone compound obtained by isolation process was determined by liquid chromatography-mass spectrometry(LC-MS)and nuclear magnetic resonance(NMR).The optimization of extraction conditions were predicted from results of orthogonal experiments,and the optimized conditions for the lactone compound with the yield of 5.91% were as follows:extractive temperature of 30 ℃,duration of 50 min,liquid to solid ratio of 15 mL/g,particle size of >150-180 μm and pH of 13.0.The compounds prepared under the optimized conditions were further isolated,its structural formula was proved to be levistolide A.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-07-14
基金项目:上海市中医临床重点实验室开放课题(14DZ2273200)
作者简介:徐婷(1991—),女,山东威海人,硕士,主要研究方向为天然产物的分离与纯化; 韩伟(联系人),教授,E-mail:whan@ecust.edu.cn.
引用本文:徐婷,王俊超,赵少甫,等.川芎中欧当归内酯A的提取纯化与结构表征[J].南京工业大学学报(自然科学版),2018,40(2):71-76..
更新日期/Last Update: 2018-03-30