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[1]汪佳丹,徐婷,韩伟.金针菇中总多酚含量测定方法的优选[J].南京工业大学学报(自然科学版),2017,39(02):113-120.[doi:10.3969/j.issn.1671-7627.2017.02.019]
 WANG Jiadan,XU Ting,HAN Wei.Optimization on determination of total polyphenol in Flammulina velutipes[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2017,39(02):113-120.[doi:10.3969/j.issn.1671-7627.2017.02.019]
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金针菇中总多酚含量测定方法的优选()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
39
期数:
2017年02期
页码:
113-120
栏目:
出版日期:
2017-03-20

文章信息/Info

Title:
Optimization on determination of total polyphenol in Flammulina velutipes
文章编号:
1671-7627(2017)02-0113-08
作者:
汪佳丹徐婷韩伟
华东理工大学 药学院 中药现代化工程中心,上海 200237
Author(s):
WANG JiadanXU TingHAN Wei
Engineering Center for Traditional Chinese Medicine Modernization,School of Pharmacy, East China University of Science and Technology,Shanghai 200237,China
关键词:
金针菇 多酚 含量测定 二次正交旋转组合设计
Keywords:
Flammulina velutipes polyphenol content determination quadratic orthogonal rotation combination design
分类号:
R284.1
DOI:
10.3969/j.issn.1671-7627.2017.02.019
文献标志码:
A
摘要:
为优选金针菇中总多酚含量测定的方法和条件,以方法学验证结果等作为评价指标,考察福林酚法、紫外分光光度法及普鲁士蓝法测定金针菇多酚含量的适用性,选择最佳测定方法; 再以吸光度为响应值,对优选方法显色条件进行单因素考察,在此基础上采用二次正交旋转组合设计进行优化。结果表明:综合比较3种方法干扰性、灵敏度及稳定性等,福林酚法更适用于金针菇总多酚含量的测定; 条件优化后最佳显色参数为福林酚显色剂2 mL,15%Na2CO3溶液2.4 mL,时间70 min,温度45 ℃,且该方法条件优化结果拟合度高,稳定可靠,可大大提高测定金针菇总多酚的灵敏度。
Abstract:
To optimize the conditions to determinate the total polyphenol in Flammulina velutipes with validation results and other evaluating indicators,the applicability of Folin-Ciocalteu method,UV spectrophotometry and Prussian blue method was investigated,and the method for determination of total polyphenol in Flammulina velutipes was selected.With the absorbance as indicator,single-factor experiments of chromogenic conditions were studied,and the chromogenic conditions were optimized by quadratic orthogonal rotation combination design.Results showed that comparing the interference,sensitivity and stability of the three methods,Folin-Ciocalteu method was the optimal one for the determination of total polyphenol in Flammulina velutipes.The optimum chromogenic conditions were 2 mL Folin-Ciocalteu reagent,2.4 mL 15%Na2CO3 at 45 ℃ for 70 min.The method was accurate,stable and reliable for the determination of total polyphenol in Flammulina velutipes with greatly improved detection sensitivity.

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相似文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-12-04
基金项目:上海市科技兴农重点攻关项目[沪农科攻字(2012)第2-9号]
作者简介:汪佳丹(1990—),女,浙江萧山人,硕士,主要研究方向为天然产物的分离与纯化; 韩伟(联系人),教授,E-mail:whan@ecust.edu.cn.
引用本文:汪佳丹,徐婷,韩伟.金针菇中总多酚含量测定方法的优选[J].南京工业大学学报(自然科学版),2017,39(2):113-120..
更新日期/Last Update: 2017-03-20