|本期目录/Table of Contents|

[1]刘贵友,万俊,肖文.乳源β-Casein-125肽的基本特征及促B淋巴细胞增殖作用[J].南京工业大学学报(自然科学版),2016,38(06):130-134.[doi:10.3969/j.issn.1671-7627.2016.06.022]
 LIU Guiyou,WAN Jun,XIAO Wen.Basic characteristics of a human milk-derived peptide β-Casein-125 and its effect on B lymphocyte proliferation[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2016,38(06):130-134.[doi:10.3969/j.issn.1671-7627.2016.06.022]
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乳源β-Casein-125肽的基本特征及促B淋巴细胞增殖作用()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
38
期数:
2016年06期
页码:
130-134
栏目:
出版日期:
2016-11-15

文章信息/Info

Title:
Basic characteristics of a human milk-derived peptide β-Casein-125 and its effect on B lymphocyte proliferation
文章编号:
1671-7627(2016)06-0130-05
作者:
刘贵友1万俊2肖文1
1.江苏第二师范学院 生命科学与化学化工学院,江苏 南京 210013; 2.南京市妇幼保健院,江苏 南京 210004
Author(s):
LIU Guiyou1WAN Jun2XIAO Wen1
1.College of Life Science and Chemistry,Jiangsu Second Normal University,Nanjing 210013,China; 2.Nanjing Maternity and Child Health Care Hospital,Nanjing 210004,China
关键词:
母乳 β-Casein-125肽 B淋巴细胞 增殖
Keywords:
human milk β-Casein-125 peptide B lymphocyte proliferation
分类号:
Q71
DOI:
10.3969/j.issn.1671-7627.2016.06.022
文献标志码:
A
摘要:
利用Uniprot、SABLE、ProtParam tool等在线数据库分析β-Casein-125肽生物学特征; 利用质谱定量技术检测β-Casein-125肽在初乳、过渡乳和成熟乳中的含量变化; 利用细胞增殖实验方法揭示β-Casein-125肽在促进B淋巴细胞增殖中的作用。结果表明:β-Casein-125肽的稳定性系数为111.43,脂肪指数和亲水性分别为111.43和0.471,属于疏水性稳定型多肽。质谱定量分析发现:β-Casein-125肽含量随泌乳时间变化含量显著降低。β-Casein-125肽不仅可以顺利进入小鼠B淋巴细胞,并且可显著促进细胞增殖。β-Casein-125肽具有高稳定性和疏水性的特征,可以促进淋巴细胞增殖,提示该肽可能在新生儿免疫系统建立中发挥重要作用。
Abstract:
The biologic characteristics of β-Casein-125 peptide were analyzed by Uniprot, SABLE, ProtParam tool and other online databases. The content of β-Casein-125 in colostrum, transition milk and mature milk was mesured by mass spectrometry technology. Furthermore, the effect of β-Casein-125 peptide in B lymphocyte proliferation was evaluated by MTT assay. The online databases showed that stability coefficient, aliphatic index and grand average of hydropathicity of β-Casein-125 were 111.43, 111.43 and 0.471, respectively. It indicated that β-Casein-125 is a hydrophobic stable peptide. The content of β-Casein-125 was significantly decreased with lactation time, detected by mass spectrometry technology. Moreover, β-Casein-125 could across the cell membrane of mouse B lymphocytes, and promote their proliferation. β-Casein-125, a hydrophobic peptide with high stability, could promote lymphocyte proliferation. It was indicated that β-Casein-125 played a critial role in promoting the neonatal immune system maturation.

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备注/Memo

备注/Memo:
收稿日期:2016-06-16
基金项目:江苏省自然科学基金(BK20151466)
作者简介:刘贵友(1973—),男,江苏江宁人,副教授,博士,主要研究方向为生物活性肽及其健康效应; 肖文(联系人),讲师,E-mail:haxiaowen@163.com.
引用本文:刘贵友,万俊,肖文.乳源β-Casein-125肽的基本特征及促B淋巴细胞增殖作用[J].南京工业大学学报(自然科学版),2016,38(6):130-134..
更新日期/Last Update: 2016-12-20