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[1]谭长强,罗晨曦,黄和.同种螺旋神经节和蜗神经抗原免疫致豚鼠听神经病样病变的抗原成分分析[J].南京工业大学学报(自然科学版),2016,38(01):103-110.[doi:10.3969/j.issn.1671-7627.2016.01.017]
 TAN Changqiang,LUO Chenxi,HUANG He.Antigen composition analysis on guinea pig models with auditory neuropathy-induced by spiral ganglion and cochlear nerve antigen immunization[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2016,38(01):103-110.[doi:10.3969/j.issn.1671-7627.2016.01.017]
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同种螺旋神经节和蜗神经抗原免疫致豚鼠听神经病样病变的抗原成分分析()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
38
期数:
2016年01期
页码:
103-110
栏目:
出版日期:
2016-01-10

文章信息/Info

Title:
Antigen composition analysis on guinea pig models with auditory neuropathy-induced by spiral ganglion and cochlear nerve antigen immunization
文章编号:
1671-7627(2016)01-0103-08
作者:
谭长强12罗晨曦1黄和1
1.南京工业大学 药学院 材料化学工程国家重点实验室,江苏 南京 210009; 2.南京大学医学院附属医院暨南京鼓楼医院 耳鼻咽喉-头颈外科,江苏 南京 210008
Author(s):
TAN Changqiang12LUO Chenxi1HUANG He1
1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Pharmacy, Nanjing Tech University, Nanjing 210009, China; 2. Department of Otolaryngology & Head and Neck Surgery, Nanjing Drum Tower Hospital and Affiliated Hospital of Na
关键词:
内耳组织抗原 听神经病 内耳自身免疫 免疫印迹法
Keywords:
inner ear tissue antigen auditory neuropathy inner ear autoimmune Western blotting
分类号:
R764.35
DOI:
10.3969/j.issn.1671-7627.2016.01.017
文献标志码:
A
摘要:
用同种螺旋神经节和蜗神经抗原免疫19只豚鼠,依据听觉电生理测试和DP OAE试验结果,判断听神经病豚鼠模型。采用豚鼠血清和Western blotting试验方法,比对分析模型豚鼠与非模型豚鼠针对组织抗原中不同蛋白条带抗体反应情况,探寻可能的致病抗原成分。结果表明:6只豚鼠出现听神经病样表现,Western blotting结果均出现针对相对分子质量3.1×104蛋白条带阳性反应。内耳病理观察亦显示其主要免疫炎性损伤部位位于螺旋神经节。实验提示相对分子质量3.1×104蛋白成分可能是导致自身免疫性听神经病的主要内耳组织抗原成分。
Abstract:
19 guinea pigs were immunized with allogenic spiral ganglion and cochlear nerve antigens, and auditory neuropathy(AN)animal models were evaluated based on the parameters of auditory electrophysiological and distortion product otoacoustic emission(DP OAE)tests. Western blotting test was performed with animal serum, and the different antibodies against corresponding ingredient of spiral ganglion and cochlear nerve antigens were compared between animal models and controls. The pathogenic composition of antigens could be found. Results showed that AN disorder occurred in 6 guinea pigs, and all of them appeared the positive immune response against relative molecular mass 3.1×104 protein band appeared in the Western blotting test. The histopathological observation of inner ear also showed that the main immune inflammatory injury was located in spiral ganglion, and that relative molecular mass 3.1×104 protein was main composition of antigens in the inner ear tissue associated with autoimmune AN. The result would provide the possible assessment for preparing AN animal model and clinical diagnosing autoimmune AN.

参考文献/References:

[1] STARR A,PICTON T W,SININGER Y,et al.Auditory neuropathy[J].Brain,1996,119:741.
[2] NARNE V K,PRABHU P P,CHATNI S.Time-frequency analysis of transient evoked-otoacoustic emissions in individuals with auditory neuropathy spectrum disorder[J].Hearing research,2014,313:1.
[3] DECLAU F,BOUDEWYNS A,VAN DEN ENDE J,et al.Auditory neuropathy:a challenge for diagnosis and treatment[J].B-ENT,2013(Suppl 21):65.
[4] MOHAMMADI A,WALKER P,GARDNER-BERRY K.Unilateral auditory neuropathy spectrum disorder:retrocochlear lesion in disguise?[J].Journal of larygology and otology,2014,18:1.
[5] MENTEN K,VAN EYNDE C,DESLOOVERE C,et al.Auditory neuropathy/dyssynchrony revisited[J].B-ENT,2014,10(2):105.
[6] 张娇,张秋静,王大勇,等.听神经病谱系障碍患者的内科药物综合治疗及疗效分析[J].临床耳鼻咽喉头颈外科杂志,2014,28(8):544.
[7] STARR A,SININGER Y S,PRATT H.The varieties of auditory neuropathy[J].Journal of basic clinical physiology and pharmacology,2000,11(3):215.
[8] 王璟,董明敏,迟放鲁.自身免疫性听神经病豚鼠模型的建立及听性脑干反应和畸变产物耳声发射的变化[J].中华耳鼻咽喉头颈外科杂志,2006,41(2):132.
[9] 杜延顺,赵丽萍,陈秀伍,等.抗内耳结缔组织抗体与听神经病[J].中国耳鼻咽喉头颈外科,2007,14(9):527.
[10] 谭长强,曹银成,何学勤,等.不同内耳组织抗原免疫致自身免疫性感音神经性聋的研究[J].临床耳鼻咽喉科杂志,1998,12:370.
[11] GILELS F,PAQUETTE S T,ZHANG J,et al.Mutation of foxo3 causes adult onset auditory neuropathyand alters cochlear synapse architecture in mice[J].Journal of neuroscience,2013,33(47):18409.
[12] YUAN Y,SHI F,YIN Y,et al.Ouabain-induced cochlear nerve degeneration:synaptic loss and plasticity in a mouse model of auditory neuropathy[J].Journal of association for research in otolaryngology,2014,15(1):31.
[13] YE H B,SHI H B,WANG J,et al.Bilirubin induces auditory neuropathy in neonatal guinea pigs via auditory nerve fiber damage[J].Journal of neuroscience research,2012,90(11):2201.
[14] FU Y,DING D,JIANG H,et al.Ouabain-induced cochlear degeneration in rat[J].Neurotoxicity research,2012,22(2):158.
[15] MAY B J,LAUER A M,ROOS M J.Impairments of the medial olivocochlear system increase the risk of noise-induced autory neuropathy in laboratory mice[J].Otology and neurotology,2011,32(9):1568.
[16] COWPER-SMITH C D,DINGLE R N,GUO Y,et al.Synchronous auditory nerve activity in the carboplatin-chinchilla model of auditory neuropathy[J].International journal of pediatric otorhinolaryngology,2008,72(8):1135.
[17] 谭长强,钟启明,何学勤,等.内淋巴囊局部同种内耳抗原免疫诱发自身免疫性感音神经性聋的实验研究[J].临床耳鼻咽喉科杂志,1993(7):153.
[18] 谭长强,曹银成,刘桦,等.内淋巴囊同种抗原免疫致自身免疫性Meniere病的实验研究[J].耳鼻咽喉-头颈外科,1997,4(1):47.
[19] MATSUOKA H,CHENG K C,KRUG M S,et al.Murine model of autoimmune hearing loss induced by myelin protein P0[J].Ann otol rhinol laryngol,1999,108(3):255.
[20] GONG S S,YU D Z,WANG J B.Relationship between three inner ear antigens with different molecular weights and autoimmune inner ear disease[J].Acta otolaryngol,2002,122:5.

备注/Memo

备注/Memo:
收稿日期:2014-09-05
基金项目:国家自然科学基金(81070789)
作者简介:谭长强(1962—),男,江苏淮安人,主任医师,博士,主要研究方向为内耳疾病; 罗晨曦为并列第一作者; 黄和(联系人),教授,E-mail: biotech@njtech.edu.cn.
引用本文:谭长强,罗晨曦,黄和.同种螺旋神经节和蜗神经抗原免疫致豚鼠听神经病样病变的抗原成分分析[J].南京工业大学学报(自然科学版),2016,38(1):103-110..
更新日期/Last Update: 2016-01-20