|本期目录/Table of Contents|

[1]韩伟,刘曦.枇杷叶三萜酸的大孔树脂分离纯化工艺[J].南京工业大学学报(自然科学版),2014,36(06):66-71.[doi:10.3969/j.issn.1671-7627.2014.06.012]
 HAN Wei,LIU Xi.Separation and purification of triterpenic acid from Eribotrya japonica leaf by macroporous resins[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2014,36(06):66-71.[doi:10.3969/j.issn.1671-7627.2014.06.012]
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枇杷叶三萜酸的大孔树脂分离纯化工艺()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
36
期数:
2014年06期
页码:
66-71
栏目:
出版日期:
2014-11-21

文章信息/Info

Title:
Separation and purification of triterpenic acid from Eribotrya japonica leaf by macroporous resins
文章编号:
1671-7627(2014)06-0066-06
作者:
韩伟刘曦
华东理工大学 中药现代化工程中心,上海 200237
Author(s):
HAN WeiLIU Xi
Engineering Center for Tradition Chinese Medicine Moderniziation, East China University of Science and Technology,Shanghai 200237,China
关键词:
枇杷叶 三萜酸 大孔树脂 分离纯化
Keywords:
Eribotrya japonica leaf triterpenic acid macroporous resins separation and purification
分类号:
R284.2
DOI:
10.3969/j.issn.1671-7627.2014.06.012
文献标志码:
A
摘要:
以枇杷叶为研究对象,采用大孔吸附树脂对枇杷叶三萜酸的粗提物进行分离纯化。首先对8种大孔树脂进行筛选,然后考察最佳大孔树脂对枇杷叶三萜酸的静态、动态吸附及脱附性能,得到最佳分离纯化的工艺条件:大孔树脂型号为HZ-816,上样流速2 BV/h(1 BV约为32 mL),上样质量浓度0.6 mg/mL,上样体积470 mL,洗脱液乙醇体积分数95%,洗脱流速2 BV/h,洗脱剂的用量为6 BV,由此得到的三萜酸纯度为92.29%。通过比较研究表明大孔树脂分离法优于碱溶酸沉法。
Abstract:
Triterpenic acid in Eribotrya japonica leaf was isolated and purified with macroporous resin.Its adsorption and desorption characteristics on eight macroporous resins with different particle sizes were investigated both in a static and dynamic way.The effects of the sample flow rate,sample concentration,sample volume,concentration of ethyl alcohol as eluent,eluent flow rate,and eluent volume were investigated to acquire the optimum isolation and purifying conditions.Triterpenic acid could be well isolated and purified with HZ-816 macroporous resin at a sample flow rate of 2 BV/h(1 BV≈32 mL),sample concentration of 0.6 mg/mL,sample volume of 470 mL,95% ethyl alcohol as eluent,eluent flow rate of 2 BV/h,and eluent volume of 6 BV.The purified triterpenic acid reached a purity of 92.29%.The method for macroporous resins adsorption was better than the method for alkali dissolving acid precipitation.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-11-29
作者简介:韩伟(1968—),男,江苏宝应人,教授,博士,主要研究方向为传质与分离工程、中药制药工程,E-mail:whan@ecust.edu.cn..
更新日期/Last Update: 2014-11-20