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[1]杨冬青,余定华,贾儒,等.基于异染原理研究聚天冬氨酸与喹哪啶红的作用[J].南京工业大学学报(自然科学版),2014,36(06):54-58.[doi:10.3969/j.issn.1671-7627.2014.06.010]
 YANG Dongqing,YU Dinghua,JIA Ru,et al.Interaction of polyaspartrate and dye probe quinaldine red from metachromasy principle[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2014,36(06):54-58.[doi:10.3969/j.issn.1671-7627.2014.06.010]
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基于异染原理研究聚天冬氨酸与喹哪啶红的作用()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
36
期数:
2014年06期
页码:
54-58
栏目:
出版日期:
2014-11-21

文章信息/Info

Title:
Interaction of polyaspartrate and dye probe quinaldine red from metachromasy principle
文章编号:
1671-7627(2014)06-0054-05
作者:
杨冬青余定华贾儒赵锦波黄和
南京工业大学 生物与制药工程学院 材料化学工程国家重点实验室,江苏 南京 211800
Author(s):
YANG DongqingYU DinghuaJIA RuZHAO JinboHUANG He
State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China
关键词:
聚天冬氨酸 喹哪啶红 异染原理 热力学参数
Keywords:
polyaspartic acid quinaldine red metachromasy thermodynamic parameter
分类号:
TQ013
DOI:
10.3969/j.issn.1671-7627.2014.06.010
文献标志码:
A
摘要:
基于异染原理研究聚天冬氨酸钠(PAspNa)与2-[4-(二甲基氨基)苯乙烯]-1-乙基喹啉鎓碘化物(喹哪啶红,QR)的相互作用。利用紫外可见分光光度测定法研究QR光谱的变化,考察了离子强度、醇类和尿素等对异染效应的影响,PAspNa与QR结合常数与热力学参数通过异染光谱的吸光度测定。结果表明QR与PAspNa相互作用后光谱发生了剧烈变化,说明加入PAspNa改变了溶液环境导致了异染现象。另外,外添加物如离子强度、醇类和尿素等对异染的破坏反映了异染结合物的稳定性并间接反映了二者的结合机制。PAspNa与QR相互作用不仅可以模拟PAspNa与药物的结合方式,还可以作为一种新的模型用QR作为探针检测生物大分子。
Abstract:
The interaction of sodium polyaspartate(PAspNa)and 2-[4-(dimethylamino)phenyl-ethenyl]-1-ethyl-quinoliniuiodide(quinaldine red,QR)from the metachromasy principle was investigated.The change of QR spectrum was studied by uv-vis spectrophotometry,and the ionic strength,alcohols and urea were inspected to study the influence on metachromasy.The interaction constants and thermodynamic parameters were determined by absorbance measurements.Results showed that the dramatic change in the spectrum of PAspNa-interacting QR indicated the presence of a completely different environment compared with that in water.The factors,such as ionic strength,alcohols and urea,on the reversal of metachromasy were discussed,and were used for determining the stability of the metachromatic complex as well as for understanding the nature of binding.QR that was strongly bound to PAspNa could be used to model the interaction between PAspNa and drugs, and as a new model to detect biomacromolecules.

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备注/Memo

备注/Memo:
收稿日期:2014-02-20
基金项目:国家科技部支撑计划(2012BAD32B00)
作者简介:杨冬青(1989—),女,江苏徐州人,硕士,主要研究方向为聚天冬氨酸合成应用; 余定华(联系人),副教授,E-mail: yudh@njtech.edu.cn..
更新日期/Last Update: 2014-11-20