|本期目录/Table of Contents|

[1]韩伟,张玲玲,黄兮,等.桑葚多糖的絮凝纯化及其抗氧化性[J].南京工业大学学报(自然科学版),2014,36(01):97-101.[doi:10.3969/j.issn.1671-7627.2014.01.018]
 HAN Wei,ZHANG Lingling,HUANG Xi,et al.Flocculating purification and oxidation resistance of mulberry polysaccharides[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2014,36(01):97-101.[doi:10.3969/j.issn.1671-7627.2014.01.018]
点击复制

桑葚多糖的絮凝纯化及其抗氧化性()
分享到:

《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
36
期数:
2014年01期
页码:
97-101
栏目:
出版日期:
2014-01-20

文章信息/Info

Title:
Flocculating purification and oxidation resistance of mulberry polysaccharides
文章编号:
1671-7627(2014)01-0097-05
作者:
韩伟张玲玲黄兮邱绍兵吕剑昆
华东理工大学 中药现代化工程中心,上海 200237
Author(s):
HAN WeiZHANG LinglingHUANG XiQIU ShaobingLÜ Jiankun
Engineering Center for Traditional Chinese Medicine Modernization, East China University of Science and Technology,Shanghai 200237,China
关键词:
桑葚多糖 絮凝纯化 抗氧化性
Keywords:
mulberry polysaccharides flocculating purification oxidation resistance
分类号:
R284.2
DOI:
10.3969/j.issn.1671-7627.2014.01.018
文献标志码:
A
摘要:
采用ZTC-Ⅱ型絮凝剂纯化经微波协同提取的桑葚多糖提取液。通过实验考察药液浓缩比、转速、絮凝剂用量和絮凝温度等因素对纯化效果的影响,结合正交试验进行参数优化。结果表明:在最优的纯化工艺条件药液浓缩比15 mL/g、转速900 r/min、絮凝剂用量16%下,蛋白质除去率为30.14%,多糖损失率为2.21%; 以半数抑制率(IC50)为指标,桑葚多糖的自由基清除率优于抗坏血酸和芦丁。
Abstract:
Mulberry polysaccharide extracted with the microwave extraction was purified by ZTC-Ⅱ flocculants.The concentrated rate,speed,amount of flocculants and temperature on the effect of purification were investigated,and the optimum conditions were obtained by orthogonal experiments.Results showed that the optimum conditions were the concentration ratio of 15 mL/g,rotational speed of 900 r/min and the adding amount of flocculants to the used concentrated solution of 16%.Under the optimum conditions,the protein clearance rate was above 30.14%,and polysaccharide loss rate was above 2.21%.Compared with ascorbic acid and ruin of half inhibiting concentration(IC50),mulberry polysaccharides showed great DPPH· scavenging activity.

参考文献/References:

[1] 王传荣.桑葚保健鲜啤酒的开发与研究[J].中国酿造,2008(7):88-90.
[2] 李颖,李庆典.桑葚多糖抗氧化作用的研究[J].中国酿造,2010(4):59-61.
[3] GAO Jing,刘培勋,LONG Wei,等.Ⅱ型ZTC1+1天然澄清剂精制仙鹤复方总糖的研究[J].中国新药杂志,2008,17(15):1335-1338.
[4] 韩伟,邱绍兵,吕剑坤,等.中药分离中的絮凝剂及其应用研究进展[J].机电信息,2012(17):1-5.
[5] 魏秀娟,向发椿,崔明筠,等.铁苋菜多糖提取工艺优化[J].安徽农业科学,2012,40(34):16548-16551.
[6] 董英,张艳芳,孙艳辉.水飞蓟粗多糖脱蛋白方法的比较[J].食品科学,2007,28(12):82-84.
[7] Ostrakhovich E A,Carles C.Evaluation of scavenging activity assessed by Co(Ⅱ)/EDTA-induced luminol chemiluminescence and DPPH·(2,2-diphenyl-1-picrylhydrazyl)free radical assay[J].Journal of Pharmacological and Toxicological Methods,2000,44(3):507-512.
[8] 张改平,朱华泽,杨建雄,等.甘草提取物的体外抗氧化活性[J].生物加工过程,2010,8(3):53-57.
[9] 张咏梅,赵振彪.ZTC-Ⅲ型天然澄清剂在中风安口服液制备中的应用[J].中成药,2004,26(4):337-338.
[10] 汤淏,唐安福,崔恩忠,等.ZTC1+1天然澄清剂在中药制剂工艺应用中的影响因素分析[J].医学研究生报,2012,25(3):324-326.

备注/Memo

备注/Memo:
收稿日期:2013-03-12
基金项目:国家大学生创新计划(111025124)
作者简介:韩伟(1968—),男,江苏宝应人,教授,博士,主要研究方向为传质与分离工程、中药制药工程,E-mail:whan@ecust.edu.cn..
更新日期/Last Update: 2014-01-20