|本期目录/Table of Contents|

[1]孟小琴,胡永红,杨文革,等.HPLC法检测发酵液中赤藓糖醇含量[J].南京工业大学学报(自然科学版),2013,35(01):1-105.[doi:10.3969/j.issn.1671-7627.2013.01.022]
 MENG Xiaoqin,HU Yonghong,YANG Wenge,et al.Determination of erythritol in fermentation broth by HPLC[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2013,35(01):1-105.[doi:10.3969/j.issn.1671-7627.2013.01.022]
点击复制

HPLC法检测发酵液中赤藓糖醇含量()
分享到:

《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
35
期数:
2013年01期
页码:
1-105
栏目:
出版日期:
2013-01-20

文章信息/Info

Title:
Determination of erythritol in fermentation broth by HPLC
文章编号:
1671-7627(2013)01-0105-04
作者:
孟小琴1胡永红1杨文革1唐容容1沈飞2
1. 南京工业大学 生物与制药工程学院,江苏 南京 210009; 2. 南京新百药业有限公司,江苏 南京 210038
Author(s):
MENG Xiaoqin1 HU Yonghong1 YANG Wenge1 TANG Rongrong1 SHEN Fei2
1. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China; 2. Nanjing Xinbai Pharmaceutical Co., Ltd., Nanjing 210038, China
关键词:
赤藓糖醇 高效液相色谱法 发酵液 含量测定
Keywords:
erythritol HPLC fermentation broth quantitative determination
分类号:
R917
DOI:
10.3969/j.issn.1671-7627.2013.01.022
文献标志码:
A
摘要:
建立用高效液相色谱法(HPLC)测定发酵液中赤藓糖醇含量方法。采用Agilent NH2(4.6 mm×250 mm,5 μm)色谱柱,以V(乙腈):V(水)=75:25为流动相,柱温35 ℃,流速1.0 mL/min,检测器为示差折光检测器,进样量20 μL。赤藓糖醇标准品质量浓度在0.2~10 mg/mL范围内线性关系良好,线性回归方程为Y=2.677 28X+0.218 88,相关系数r=0.999 23,相对标准偏差(RSD)=1.66%,试样回收率为98.1%~104.0%。实验结果表明该方法简便、快速、结果可靠。
Abstract:
High performance liquid chromatography(HPLC)for the quantitative determination of erythritol in the fermentation broth was presented on a Agilent NH2 HPLC column(4.6 mm×250 mm,5 μm). The mobile phase was acetonitrile-water of 75:25 with the flow rate of 1.0 mL/min and the column temperature of 35 ℃. The refractive index detector was used. The results showed that the external standard calibration curve for quantifying erythritol by HPLC was validated from 0.2 mg/mL to 10 mg/mL with a satisfied linear relationship.The linear regression equation was Y=2.677 28X+0.218 88, and the correlation coefficient was 0.999 23. The recovery rate of the erythritol was 98.1%-104% with the relative standard deviation(RSD)of 1.66%. The experimental results showed that this method is simple, efficient and reliable.

参考文献/References:

[1] 庞明利,杨海军.赤藓糖醇的功能研究及应用分析[J].食品安
  全导刊,2011(6):64-65.
[2] Waldemar R,Anita R,Marta M.High-yield production of erythritol from raw glycerol in fed-batch cultures of Yarrowia lipolytica[J].Biotechnol Lett,2009,31:377-380.
[3] 肖素荣,李京东.赤藓糖醇的特性及应用[J].中国食物与营养,2008(5):26-28.
[4] Hee-Jung M,Marimuthu J,In-Won K,et al.Biotechnological production of erythritol and its applications[J].Applied Microbiology and Biotechnology,2010,86:1017-1025.
[5] Sawada K,Taki A,Yamakawa T,et al.Key role for transketolase activity in erythritol production by Trichosporonoides megachiliensis SN-G42[J].Journal of Bioscience and Bioengineering,2009,108(5):385-390.
[6] Hao Hongxun,Hou Baohong,Wang Jingkang,et al.Solubility of erythritol in different solvents[J].Journal of Chemical and Engineering Data,2005,50(4):1454-1456.
[7] 董海洲,叶娴,侯汉学.Torulopsis sp.ERY237 产赤藓糖醇工艺条件的研究[J].食品与发酵工业,2008,34(4):75-79.
[8] 杨文革,胡永红,李玲.一种酵母及其多阶段发酵生产赤藓糖醇的方法:中国,201010593775.6[P].2010-12-17.
[9] 胡永红,杨文革,孟小琴.一种发酵液中分离纯化赤藓糖醇的方法:中国,201110200325.0[P].2011-07-18.

备注/Memo

备注/Memo:
收稿日期:2011-11-25
基金项目:国家自然科学基金(31171644); 江苏省科技支撑(农业)计划(BE2011354)
作者简介:孟小琴(1988—),女,江苏南通人,硕士生,主要研究方向为微生物发酵; 胡永红(联系人),教授,E-mail: yonghonghu09@gmail.com..
更新日期/Last Update: 2013-01-20