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Biomineral synthesis of nano-selenium with Escherichia coli MG1655 as intermediary(PDF)


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Biomineral synthesis of nano-selenium with Escherichia coli MG1655 as intermediary
WU Tong12WANG Zhenjiong2HUA Chun2ZHOU Feng2WANG Renlei3HUANG He1
1. College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China; 2. School of Food Science,Nanjing Xiaozhuang College,Nanjing 211171,China; 3. Biology Department,Jiangsu Second Normal University,Nanjing 210013,China
Escherichia coli MG1655 sodium selenite nano-selenium characterization
The reduction with Escherichia coli MG1655 of sodium selenite was used to prepare nano-selenium with different mass concentrations of sodium selenite as selenium source artificially.The influence of sodium selenite mass concentration on the growth curve of Escherichia coli MG1655 was investigated.The reduction efficiency of sodium selenite was determined by hydride generation atomic fluorescence spectrometry(HG-AFS),and the structure of the product was characterized by scanning electron microscope(SEM),X-ray diffraction(XRD)and Fourier transform infrared spectroscopy(FT-IR).Results indicated that sodium selenite could be biomineralized in abundance by Escherichia coli MG1655 to nano-selenium particles in red simple substance with their diameters mainly 100~250 nm.Moreover,all the reduction rates of various mass concentrations of sodium selenite solution were over 50%.


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Last Update: 2017-05-31